FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735673
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18300.24875182316056182No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAT10850.147484004442191No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC10560.14354203565986517No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC9420.12804602044658428No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC8990.12220103225210113No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC7760.1054816474167191No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACTA305.9219997E-446.594911
TTACTCG305.931522E-446.5797164
CGTAAAT450.00432046531.09113523
TGTTGAA952.7866008E-729.51494662
AATCTAG604.929159E-429.21944269
TCTAGGC605.036298E-429.1123223
GTGTAGG1004.4916305E-727.9478281
AATTAGT1653.6379788E-1227.5805531
ATTGTTG906.102655E-627.26033260
CGAGTAA657.87149E-426.9717970
CAAGTTA657.8978506E-426.95636253
TAGGCCG1705.456968E-1226.7148365
CTATAGG808.774799E-526.201091
TTAGTGA1757.2759576E-1226.00451933
CTTAAGG2050.025.5620383
AAATCTA1101.0011481E-625.49643168
ACAAGAG1801.0913936E-1125.32351569
AGAGTGA1251.1184602E-725.1530466
ATGCACT700.001239303424.9534194
TTTCGGA1301.634271E-724.1856211
Produced by FastQC (version 0.11.2)