FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62174
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34805.597194968958085No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5900.9489497217486409No Hit
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1730.2782513590890083No Hit
CTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAG850.13671309550616012No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA840.13510470614726414No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT830.13349631678836812No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG770.12384598063499212No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT700.11258725512272011No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG660.1061536976871361No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG640.1029369189693441No Hit
ATATTAAATAGATATCTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTT640.1029369189693441No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC630.10132852961044811No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGAC150.002192365270.1637560
TCACAAG150.002226508769.89126621
AAGGACG150.002226508769.89126625
ATTGTAC150.002235106269.823473
ATTAGGT200.006850832652.6228164
CGACAAT200.006877314852.57156852
GCCAATT200.006877314852.57156845
ATAGCAA200.006903873352.5204336
GGGCACC200.006903873352.5204340
CGTGCGC200.006930507752.46938730
GTGCGCC200.006930507752.46938731
CCAATGA200.006957218552.4184516
AGGACGT200.006957218552.4184526
AATTGTC200.006957218552.4184527
CACAAGG200.006957218552.4184522
AGGACAA200.006984005752.3676075
GTACTTG200.006984005752.3676076
GATCTGG503.476247E-641.894086
GTGCTCT602.5866757E-740.730361
TGCTCTT602.5866757E-740.730362
Produced by FastQC (version 0.11.2)