FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126661
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23961.8916635744230663No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20811.642968238052755No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11510.9087248640070741No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4010.31659311074442803No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2890.22816810225720624No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2290.18079756199619457No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2210.174481489961393No Hit
GATCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2000.15790180087003894No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1880.14842769281783658No Hit
TCCGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1790.14132211177868484No Hit
CTCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1720.13579554874823346No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT1520.12000536866122959No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1440.11368929662642802No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAG150.002231583269.9018310
TCCCGAG150.002231583269.901832
GGGCAAT150.002231583269.9018316
ATATTCC301.0250551E-769.9018312
CATAGAA252.3974592E-455.9214672
TTAGGGA200.006972933752.426373
CTGTCCT200.006972933752.426379
GATAATA200.006972933752.426378
GCACGGC200.006972933752.426376
TTATCTT200.006972933752.4263714
TCTACGA200.006972933752.426378
CTAGTAC200.006972933752.426379
AGGGGTT200.006972933752.426375
TCTTTGC200.006972933752.426378
CGATCAA200.006972933752.426375
AAAGCGA200.006972933752.4263723
TCTGGCC352.0915557E-549.9298748
CGCAGGG404.6038498E-543.6886412
CTAAGGC350.001259164339.94394
TGACGCG350.001259164339.943910
Produced by FastQC (version 0.11.2)