FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102360
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGACCTGTCGGC26920.24420334554954826No Hit
GGTGTGCACCGGTCGTCTCGTCCCTTCTGCCGGCGATGCGCTCCTGGCCT21250.1927682426793425No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC19460.17653035306070614No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC19250.1746253492506985No Hit
GTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGC17670.16029246344206974No Hit
GACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTT16620.15076744439203163No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCT16480.14949744185202657No Hit
CCCTAACGTTGCCGTCAACCGCCGCGTCCCGGTTCAGGAATTTTAACCCG15910.144326717224863No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGG15040.13643455858340287No Hit
ACACTGATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAA14720.13353169563481984No Hit
GGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATT13730.12455096338764107No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCC13270.12037809789905295No Hit
GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTATAGCC12650.1147538009361733No Hit
CTTCCGATCTGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTT12570.11402808519902755No Hit
ACCTATACCCGGCCGTCGGGGCAAGCGCCAGGCCCCGATGAGTAGGAGGG12460.11303022606045211No Hit
GATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAATCTTT12080.10958307630900975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11540.10468449508327587No Hit
CCTATAAGCAACATCCGCCGATCCCTGGTCGGCATCGTTTATGGTTGAGA11290.1024166334046954No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT11250.10205377553612249No Hit
CATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACC11050.1002394861932581No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA459.2919574E-538.8236161
TCTGACC1700.037.000648
TCTACGT400.002437934.945058
GTATTAT400.00243919934.9412571
GTCTAGG708.812476E-734.9412541
ATCTGGG9950.034.0651867
CCTGGGT1450.033.7363853
AGGGTTA652.127871E-532.2534685
CCTATAT652.127871E-532.2534681
CGGTATG553.0207078E-431.7682299
CTAGGCC1001.2805685E-831.4471323
CCTATCC450.004343577731.0588933
TATACTG703.530344E-529.9496485
CTGAGGC1103.23289E-828.5914069
GATCTTT3800.028.5047116
TATAGGT8200.028.123453
ATTCGGC6850.028.12220247
CAGTCAC500.007150322728.04741767
CAGGCCC5250.027.99099729
GCCAGGC5000.027.98491127
Produced by FastQC (version 0.11.2)