FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944270
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52170.5524902834994229No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC16290.1725142173319072No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT16200.17156110010907896No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC14230.1506984231205058No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC13540.1433911910788228No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC11910.1261291791542673No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG10700.11331504760291018No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT10610.11236193038008198No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA10560.11183242081184407No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA10550.1117265188981965No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10320.10929077488430217No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC10070.10664322704311267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGC305.929968E-446.5838477
GAGCATA605.0240487E-946.5749931
AGCATAC654.940648E-737.6206472
TTTCGGA1800.032.990621
AAACGTA755.5329743E-528.03912261
TATACTA500.007271800727.9503088
ATATAAG2950.027.2362562
GGTCCAA3600.027.17735111
TTCGGAA2400.026.2000922
TCGGGTT700.001237604424.96037713
TAGGGTC700.00123989224.952474
TAGGGGT1401.2141754E-824.952474
GTCTTAC4950.024.698861
CCTAGGG2250.023.2889732
GTTTTAT750.001853605723.2874971
ACGTGTA7000.023.04971570
CAAGAAT4300.022.75745815
GAATCAG5400.022.69380635
GGTTGCA5750.022.48889416
AATCAGG5450.022.48560536
Produced by FastQC (version 0.11.2)