FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674187
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24250.3596924888791982No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA9840.14595357074520868No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT8840.1311208907914273No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG7300.10827856366260401No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGT553.0221645E-431.762963
ATAGGAC703.529306E-529.9479353
TAGCGTC853.999792E-628.7811789
ATCTGGG7050.028.7494627
AGACTGG808.755301E-526.2091036
TAGACTG808.7605964E-526.2067725
ACGATTC959.390116E-625.78826133
GAACGAT959.396606E-625.78596531
GATCTGG16950.025.7709926
CTATAGG959.514102E-625.7447151
TAGTGTA4050.025.10972670
GGGAACG1401.2025339E-824.97214716
ATCTGCG4250.024.6673877
CCGGGTT1001.4111722E-524.46835114
ATCTGTG4550.023.8097
GGGTTAT1202.1986161E-623.2928371
AGTGTAG2850.022.147670
CACGTTA952.7714786E-422.11406745
GGTAAGG952.8050484E-422.07474511
ATAGCGT952.806736E-422.0727838
Produced by FastQC (version 0.11.2)