FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969400
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT18550.19135547761501961No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC10110.10429131421497834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4650.034.62294439
TCGTATG4900.032.85646440
GTATGCC4950.032.52458242
TGCTTGA5350.029.43864455
GCCGTCT5800.027.75804746
GCTTGAA6100.026.96665856
CGCTAAA1850.026.4859410
GTCTTCT6000.026.2494649
TATGCCG6200.025.96720543
CGTATGC6350.025.3538141
ATGCCGT6350.025.3538144
GATCTTT24600.025.1863046
ATCTTTT21500.025.073137
CCGTCTT6600.024.92372747
TTATACT1151.495675E-624.3510914
TCTTTTT21650.024.090998
GTGCATA1752.0008883E-1024.0069331
CGATCTT48650.023.744195
CTGCTTG6850.023.50316454
ACTGTCG2103.6379788E-1223.3364628
Produced by FastQC (version 0.11.2)