FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699888
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT16640.23775232608645955No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC14500.20717600530370575No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC12960.18517248474041562No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC12000.17145600438927372No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC10240.14630912374551358No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC9350.13359280341997576Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA8820.12602016322611617Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT8410.12016208307614933No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGT400.00241475935.0118981
TCCTATA552.986306E-431.8291
GCTAACA450.004304675431.1150192
AATATAG500.007204056328.0035152
TATGCGT906.1718692E-627.2217567
CTCGTAT3850.026.36317839
ACGATAG1401.1896191E-824.99956569
TTTCGGA3100.024.8471551
TAGACCC851.3071907E-424.7089844
TTAGACA851.3071907E-424.7089844
GTATTAA1001.3940697E-524.508331
TCGTATG4200.024.16624840
GATCTTT17900.023.6621886
CTTAATC901.926184E-423.3362643
TATAACC750.001830979423.3362644
TCTAACG901.927117E-423.3345958
TACCATA750.001832502323.3329349
TACTCTA1052.0394242E-523.3329354
TCTTTTT15350.023.2585878
GTATGCC4750.022.8417142
Produced by FastQC (version 0.11.2)