FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581194
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC9560.1644889658186423No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT9490.1632845487049075No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC9090.1564021651978513No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC8320.14315357694676822No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC7180.12353878395165814No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC6940.11940935384742443Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA6380.10977401693754582Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGC305.8681646E-446.6808973
TGTAACA400.002414837735.0106742
TAAGAGT400.002414837735.0106744
TATAGAC703.4806526E-530.009152
ATAGCGT604.972413E-429.175566
ATCTTTT9050.025.1457867
TATGCCG2650.025.09335343
ATAATAT851.3052273E-424.7134153
CCGTCTT2850.024.56043647
TCTTTTT9250.024.22368
CTTTTTT9250.024.2152659
GTATGCC2750.024.18086642
CTCGTAT2800.023.74906539
TAATATT901.923298E-423.3404484
TCGTATG2950.022.54148540
GTATTAA1102.9133815E-522.2795181
GATCTTT11650.022.2385396
TCTACAC2056.002665E-1122.2018893
GCCGTCT3000.022.16579646
TAGGGGT1755.2204996E-922.0067084
Produced by FastQC (version 0.11.2)