FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529460
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT14000.2644203528122993No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT7770.14675329581082613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3450.029.41993339
ATATTAG604.975554E-429.1718221
GTCTTCT4000.028.8746549
GCCGTCT3900.028.71759846
GTATGCC3950.028.35408842
TATTAGA1253.5488483E-928.0023062
CTGAGTG755.5829223E-527.999669
TATGCCG4150.027.83098843
TCGTATG3900.027.82017340
CCGTCTT4150.026.98762547
CGATCTG26600.026.8443155
TCTGACC1056.7602014E-726.6688618
TCTAACA808.65716E-526.252162
CCGATCT69950.025.9706864
GATCTTT10850.025.8085776
TTCCGAT71400.025.7374112
CGATCTT18800.025.6936045
TTGTACC1101.0144413E-625.4566424
TCCGATC72500.025.3951933
GCTTGAA4300.025.23225256
Produced by FastQC (version 0.11.2)