FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571872
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT26020.4549969223882267No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT14780.25844944323205193No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT8250.14426305187174754Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATC200.006950604352.49934414
ACTATAC501.7086577E-435.0026253
TTTCGGA4400.033.4145161
GGCGTAT552.9887393E-431.823351
TTCTAAT450.004303246731.1161651
ATTCTTA450.004306931531.11072342
TCGTATG6350.030.86575740
TATTAAT802.4626224E-630.6299722
GATCTTT13650.030.2586776
CTCGTAT6150.030.16222439
GTATGCC6500.030.1534742
ATCTAGC2100.030.0022497
TCTTTTT13100.029.9259078
ATCTTTT13450.029.667657
TTAAGGT952.8185605E-729.4758934
CTTTTTT13600.029.0805199
CGATCTG32600.028.98995
GATCTAG4250.028.8256916
TCTTCTG6800.028.82316850
CGATCTT21050.028.7670025
Produced by FastQC (version 0.11.2)