FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567849
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT28750.5062965682778344No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT19800.34868424528351727No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA19250.3389985718034196No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA18190.3203316374599586No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT18160.31980332799740774No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC17420.3067716945878218No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG16420.2891613791694623No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC15680.2761297457598763No Hit
CTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAG14580.2567583987996809No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG14140.24900986001560274No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG13600.23950028968968864No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG12650.22277049004224714No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT12640.22259438688806357No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA12090.21290871340796583No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT11470.20199031784858298No Hit
GGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAGTAAAA11210.19741163583980953No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA10730.188958684438997No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA10710.18860647813062978No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT10650.18754985920552825No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC10600.18666934343461025No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA10560.18596493081787588No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA10350.1822667645800204No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA10140.1785685983421649No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA10040.17680756680032897No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT9680.17046785324971955No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9500.16729799647441485No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA9420.1658891712409461No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT8710.1533858472939109No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT8710.1533858472939109No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA8670.1526814346771765No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC8590.15127260944370777No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC8520.15003988736442259No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA8470.14915937159350462No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG8460.14898326843932103No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTT8360.14722223689748506No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC8120.1429957611970788No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC8100.1426435548887116No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTA7980.1405303170385085No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA7730.1361277381839186No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA7670.13507111925881707No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGT7630.13436670664208267No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC7610.13401450033371548No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA7500.13207736563769595No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG7450.13119684986677796No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA7440.13102074671259437No Hit
ATATTAAATAGATATCTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTT7420.13066854040422718No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA7290.12837919939984044No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG7290.12837919939984044No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG7090.12485713631616856No Hit
TTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGT7070.12450493000780137No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG7070.12450493000780137No Hit
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTA7050.12415272369943417No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA6990.12309610477433261No Hit
AGCTTATGTAGCCTTCATTCATGTTGGCAGTAAAATGCTTATTGTGTTTG6890.12133507323249668No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATAT6750.11886962907392633No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA6590.11605197860698882No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTG6540.11517146283607085No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA6540.11517146283607085No Hit
TCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCA6520.11481925652770367No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGT6430.11323432814005133No Hit
CCTTATAATGGGACAAACCAACAGGTACGGCTCCACTTACATAAACACAT6330.11147329659821537No Hit
GTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCT6290.11076888398148098No Hit
ATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTT6170.10865564613127786No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC6160.10847954297709428No Hit
GATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAA6130.10795123351454347No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTC6130.10795123351454347No Hit
CTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCATGGTAGTC6010.10583799566434035No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5980.10530968620178956No Hit
TCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGG5870.10337255150577002No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATG5860.10319644835158642No Hit
GTGTTTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCATTATAAGGAC5830.10266813888903563No Hit
AGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAG5790.10196372627230127No Hit
CCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACA5740.10108321050138329No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG5690.10020269473046532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA15900.041.852331
AAACCGT350.001254300440.0060426
CCGATCT16950.039.0324444
TCCGATC17100.038.894763
TTCCGAT18400.036.153132
GATCTAG1003.255991E-1035.0052876
GGGGTGA400.002418704334.9991215
TACTGTA400.002418704334.999127
TAACGTG450.00430346231.1158085
CTAACAC450.00430346231.1158083
GTATACT450.00430346231.1158084
ATATAGG703.4716202E-530.0203951
ATATAAG3750.029.876442
AACTAGC604.9818883E-429.16593425
CGATCTC5300.029.060995
GGGTAGG755.5573655E-528.0190331
TTGCTAA500.00720823127.99929422
CCGTAGA500.00720823127.9992949
TCTTCCG6900.027.395445
TATAGGG906.1526807E-627.231132
Produced by FastQC (version 0.11.2)