FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317955
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC13580.42710446446824235No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC11040.3472189460772751No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC9620.30255853815791545No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC9470.2978408894340394No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA8810.2770832350489849Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC8610.27079303675048355Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC3320.10441729175512258No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAAC352.0840658E-549.999893
GTATGAC400.00241683734.9999242
TAGGGCC552.9886607E-431.8181114
GACACTA450.00430386931.1110422
CACTAGC450.00430386931.1110424
CGCTAAT450.00430386931.1110414
TACGTCT450.00430386931.111046
CTCTATG500.007197228.0043431
ACTACTA500.007202728727.99993759
ATCTTTT11800.027.88129412
CTTTTTT12100.027.19002214
TCTTTTT12150.027.07812913
GATCTTT13150.025.81743411
TCAAAAC700.001224619624.99994557
CTACCCG700.001224619624.99994533
AGGGCGC800.00266298121.87495210
AGTGTGC800.00266298121.87495270
GTAGAGG1154.1025603E-521.3076521
CCGGGAA850.003784875620.58818814
GAGTTAA850.003784875620.58818832