Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adr3wtzt14s88.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 317955 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 1358 | 0.42710446446824235 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 1104 | 0.3472189460772751 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 962 | 0.30255853815791545 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 947 | 0.2978408894340394 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 881 | 0.2770832350489849 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 861 | 0.27079303675048355 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC | 332 | 0.10441729175512258 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATTTAAC | 35 | 2.0840658E-5 | 49.99989 | 3 |
GTATGAC | 40 | 0.002416837 | 34.999924 | 2 |
TAGGGCC | 55 | 2.9886607E-4 | 31.818111 | 4 |
GACACTA | 45 | 0.004303869 | 31.11104 | 22 |
CACTAGC | 45 | 0.004303869 | 31.11104 | 24 |
CGCTAAT | 45 | 0.004303869 | 31.11104 | 14 |
TACGTCT | 45 | 0.004303869 | 31.11104 | 6 |
CTCTATG | 50 | 0.0071972 | 28.004343 | 1 |
ACTACTA | 50 | 0.0072027287 | 27.999937 | 59 |
ATCTTTT | 1180 | 0.0 | 27.881294 | 12 |
CTTTTTT | 1210 | 0.0 | 27.190022 | 14 |
TCTTTTT | 1215 | 0.0 | 27.078129 | 13 |
GATCTTT | 1315 | 0.0 | 25.817434 | 11 |
TCAAAAC | 70 | 0.0012246196 | 24.999945 | 57 |
CTACCCG | 70 | 0.0012246196 | 24.999945 | 33 |
AGGGCGC | 80 | 0.002662981 | 21.874952 | 10 |
AGTGTGC | 80 | 0.002662981 | 21.874952 | 70 |
GTAGAGG | 115 | 4.1025603E-5 | 21.307652 | 1 |
CCGGGAA | 85 | 0.0037848756 | 20.588188 | 14 |
GAGTTAA | 85 | 0.0037848756 | 20.588188 | 32 |