FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575690
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT17030.2958189303270858No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC13300.23102711528774164No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC11050.19194358074658235No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC10730.1863850336118397No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT8980.15598672896871582RNA PCR Primer, Index 29 (95% over 23bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC8790.15268634160746236No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTCT604.972234E-429.1756824
GTATAGT1900.027.637721
ATCTGGG5350.027.4851267
CTCGTAT3700.026.48546839
TATAGTA2250.026.4526182
AGTACTA2150.026.0545625
ACTATAC700.001223604125.0077293
GTTTAGG1001.3947831E-524.5054441
GTATGCC4150.024.4568942
TTTCGGA2500.023.8052881
TGCTTGA4150.023.6135555
TATATGG901.9232137E-423.3405442
TCTCGTA4350.023.33243838
GTTCGTA901.9277485E-423.33243426
CGTATGC4550.023.07603541
CCGTCTT4100.023.04789447
TTGAAAA4750.022.84122558
TACTAAC2155.456968E-1222.797747
GTCTTCT4000.022.74912349
TAGTACT2500.022.4069234
Produced by FastQC (version 0.11.2)