FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520653
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT15180.29155694867791027No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC15080.28963628366685684No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC13230.2541039809623684No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC12280.23585766335736083No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC10080.19360303311418547No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT7060.13559894978037196RNA PCR Primer, Index 29 (95% over 23bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC5700.10947790563004534Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA5610.10774930712009725Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTT400.00241729435.002745
TTAGAGC501.7084152E-435.0027353
TCTACTA400.002418433334.9993827
CTCGTAT3950.029.23998539
TCGGTTA604.978312E-429.1689478
GTATGCC4200.028.33282942
TCGTATG4100.028.1702340
ATTGTAC500.00720405228.002193
ATAGTGA500.007207430427.99950248
TTTCGGA2050.027.3297081
GCCGTCT4300.026.85998546
TTACACT1056.760074E-726.6687534
CTTGAAA4400.026.2495357
CCGTCTT4450.025.95459447
GTCTTCT4450.025.95459449
TTCTGCT4500.025.66620852
GCTTGAA4650.025.5909456
TGCTTGA4550.025.38416355
CTTCTGC4700.025.31869751
GCTAAAT700.001225781224.99955226