Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adr3wtzt14s85.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 520653 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT | 1518 | 0.29155694867791027 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 1508 | 0.28963628366685684 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 1323 | 0.2541039809623684 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 1228 | 0.23585766335736083 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 1008 | 0.19360303311418547 | No Hit |
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT | 706 | 0.13559894978037196 | RNA PCR Primer, Index 29 (95% over 23bp) |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 570 | 0.10947790563004534 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 561 | 0.10774930712009725 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TACACTT | 40 | 0.002417294 | 35.00274 | 5 |
TTAGAGC | 50 | 1.7084152E-4 | 35.002735 | 3 |
TCTACTA | 40 | 0.0024184333 | 34.99938 | 27 |
CTCGTAT | 395 | 0.0 | 29.239985 | 39 |
TCGGTTA | 60 | 4.978312E-4 | 29.168947 | 8 |
GTATGCC | 420 | 0.0 | 28.332829 | 42 |
TCGTATG | 410 | 0.0 | 28.17023 | 40 |
ATTGTAC | 50 | 0.007204052 | 28.00219 | 3 |
ATAGTGA | 50 | 0.0072074304 | 27.999502 | 48 |
TTTCGGA | 205 | 0.0 | 27.329708 | 1 |
GCCGTCT | 430 | 0.0 | 26.859985 | 46 |
TTACACT | 105 | 6.760074E-7 | 26.668753 | 4 |
CTTGAAA | 440 | 0.0 | 26.24953 | 57 |
CCGTCTT | 445 | 0.0 | 25.954594 | 47 |
GTCTTCT | 445 | 0.0 | 25.954594 | 49 |
TTCTGCT | 450 | 0.0 | 25.666208 | 52 |
GCTTGAA | 465 | 0.0 | 25.59094 | 56 |
TGCTTGA | 455 | 0.0 | 25.384163 | 55 |
CTTCTGC | 470 | 0.0 | 25.318697 | 51 |
GCTAAAT | 70 | 0.0012257812 | 24.999552 | 26 |