FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688916
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT21730.315423070446905No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG16370.23761968077385343No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA15100.21918492239982815No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT14470.21004012100168962No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC13680.1985728303595794No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC13540.19654065227110418No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA13260.1924762960941537No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT13190.1914602070499161No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC12680.18405727258475635No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC11810.17142873732066027No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC11800.17128358174291206No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG11640.16896109249894037No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT11270.16359033612225585No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA10430.15139726759140448No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT10400.15096180085815977No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG10230.14849415603643987No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA10050.1458813556369717No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC9670.14036544368253895No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA9340.13557530961684733No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA9150.13281735363963096No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC8710.12643050821870883No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT8470.12294677435275127No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA8320.12076944068652783No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA8110.11772117355381498No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7890.11452775084335391No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC7830.11365681737686452No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA7810.11336650622136807No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA7300.10596357175620831No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT7200.10451201597872599No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC7150.10378623808998484No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC7140.10364108251223662Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA7120.10335077135674015No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC7020.10189921557925785No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA6960.10102828211276847No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATT450.0043046131.1150474
GTATTAA500.00720139228.0055751
ATTACAC500.00720394528.0035423
CTCGTAT1800.027.22171236
CATTAAT4100.025.6092844
ATATAAG2900.025.3498732
GCCGTCT2100.024.99953343
GGGGCAC851.3071533E-424.7090076
GTTAGTG851.3084423E-424.7054219
ATAGATA1601.8590072E-924.0655468
TTCGAAG3800.023.94692270
GTTAGAG3950.023.92360562
CCGTCTT2051.8189894E-1223.90199544
GTATGCC2051.8189894E-1223.90199539
TATGCCG2200.023.8631940
TAGAGGT4000.023.6245664
TAATCAC1502.4714609E-823.3362876
TTTAGGC901.9261298E-423.3362854
GAGGTTC4050.023.33289766
AGAGGTT4100.023.04835165
Produced by FastQC (version 0.11.2)