FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443253
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT14720.33209025094020794No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA12400.27974993965071865No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC11790.26598804745822363No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC10800.24365317324417432No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG10670.24072031097364258No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG10640.24004349660351987No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG9620.21703180801934788No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC9560.21567817927910246No Hit
CTTCCGATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTT8230.18567274220366248No Hit
GGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTG7490.16897798774063572No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA7320.16514270630994038No Hit
GATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGA7260.16378907756969496No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC7160.16153302966928595No Hit
GGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATG6750.15228323327760895No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG6580.14844795184691362No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC6550.1477711374767909No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAA6510.1468687183166273No Hit
TCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGG6190.1396493650353184No Hit
CTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAA6170.13919815545523662No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC6120.1380701315050321No Hit
GGGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT5990.13513726923450037No Hit
GCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATAC5860.13220440696396868No Hit
GCAATAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCT5840.13175319738388686No Hit
ACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAAC5750.12972275427351873No Hit
CATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGG5690.12836912553327331No Hit
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAA5670.12791791595319152No Hit
CCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGT5530.12475944889261889No Hit
CTACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAA5450.12295461057229168No Hit
CCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTG5290.11934493393163724No Hit
GGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATT5180.11686328124118732No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5050.11393041897065559No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5010.11302799981049198No Hit
TAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATG4900.11054634712004206No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGC4810.10851590400967394No Hit
ATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAG4670.10535743694910131No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4640.10468062257897859No Hit
TAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATT4630.1044550177889377No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4470.10084534114828327No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT404.5903915E-543.7497186
ATGTATG400.00241791234.9997753
TATTGGC501.7088561E-434.9997752
CAGTATA400.00241791234.9997754
TAGGGGT957.623385E-933.157684
CCTATAA552.9827142E-431.832341
TGAACAC450.00430576931.110915
TTTATGC450.00430576931.110912
CTATAAG703.4862096E-529.9998072
CTGTGCG703.4862096E-529.9998079
ATCTGGG7700.029.0907237
TTAGGAC1451.8189894E-1128.965333
TAGGACT1352.582965E-1028.5183354
TTTGTCA500.00720589127.9998247
GTCTCAT755.5806842E-527.9998260
TATCTCG500.00720589127.999825
TATACTT500.00720589127.9998263
GAACGTA1154.890535E-827.39112718
TCTGGGC5800.027.1549978
GTATATG657.93094E-426.9350571
Produced by FastQC (version 0.11.2)