FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479326
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT9910.20674864288605252No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA7080.14770740581566616No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG6870.14332625394825232No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6730.14040548603664313No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA6290.13122592974301417No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6250.1303914246254115No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5670.11829110042017332No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG5310.11078055436174962No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA5210.10869429156774305No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4990.10410451342092857No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4810.1003492403917167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTGT556.6869325E-638.185234
TTCTAAT654.850863E-737.7035451
CTATAGC400.002416970235.003133
ATAGTGC400.002416970235.003138
ATCTGTG1350.033.706727
TCTATTG450.004304095631.1138943
TGTAGTA604.977402E-429.1692755
TTCCGAT45150.028.994842
TCCGATC45300.028.898833
CTTCCGA45350.028.7957921
CCGATCT44650.028.5355854
CGATCTG19950.028.4235955
CACATAA500.007195760528.0083481
ATAGACA500.007203095628.0025044
TCTAGGC755.5779725E-528.0025042
TGTAGGA500.007203095628.0025042
TCTTTTT7650.026.53555113
TTTCGGA1606.548362E-1126.2578261
GATCTGG9000.026.0578846
ATCTTTT8350.025.98763712
Produced by FastQC (version 0.11.2)