FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635479
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT9680.1523260406716823No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT9500.14949353165092788No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT7500.11802120919810095No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT7210.11345772244244105No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6740.10606172666602673No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA6720.10574700344149845No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA6420.10102615507357443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAG556.6880275E-638.1871833
CCGATCT49750.035.7437134
TCCGATC51800.034.9373443
TTCCGAT52200.034.736682
CTTCCGA53750.033.66721
CGATCTG23850.032.8767365
ATCTGAG3250.031.2351597
GATCTGG12100.030.376176
TCTGAGC2050.029.0284718
TTACTCG755.5788485E-528.0039354
GATCTAG3150.027.7816816
CGATCTA10400.026.5902735
ATCTGGG6150.025.6133567
ATCTTTT10000.025.203547
CATACAG700.001224947325.0035135
GATCTGA9350.024.7093546
TAAGAGT851.3068726E-424.7093544
ATATTAG851.3075711E-424.707411
ATCTGAC3150.024.447887
CGATCTT17900.024.4447755
Produced by FastQC (version 0.11.2)