FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719193
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT12500.17380591857818417No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG11190.15559105831119047No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC10780.14989022418182602No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA9840.1368200191047466No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA9490.1319534533845574No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT9310.12945064815703156No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC8180.11373859311756371No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA8160.11346050364783862No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT8140.11318241417811352No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT7970.11081865368545021No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA7810.10859393792764947No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG7800.10845489319278691No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC7610.10581304323039852No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGT404.589906E-543.7551274
GTTATAC350.001254731740.0046923
TATGCCG1455.9480953E-1026.55114443
GTATGCC1455.9480953E-1026.55114442
GTGTAGT1251.09408575E-725.211721
GCCGTCT1601.8626451E-924.06197446
CATATAA3900.023.3441851
GTAATAT750.001827416623.3441851
ATATTAG750.001827416623.3441851
ATATAAG3900.023.337692
TCTAGGT750.001831117623.336073
TCTATAC1202.1648575E-623.3360673
ATCTGGG7400.023.178397
TATGCTT4100.023.0514835
TCTGTGT1253.0862175E-622.4026268
GATACAC952.7778E-422.1078553
TCTAACG952.7778E-422.1078558
ATAAGAA4300.021.979324
CTGCTAA1304.3237087E-621.548481
TAGTGTC2800.021.24953752
Produced by FastQC (version 0.11.2)