H3VNWAFXY_n01_adr3wtzt14s7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3VNWAFXY_n01_adr3wtzt14s7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	173191
Sequences flagged as poor quality	0
Sequence length	76
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	857	0.49482940799464176	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	599	0.3458609281082735	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	560	0.3233424369626597	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	532	0.3071753151145267	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	412	0.23788765005109966	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	400	0.23095888354475697	Illumina PCR Primer Index 10 (95% over 21bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	296	0.17090957382312014	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	278	0.16051642406360608	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT	175	0.10104451155083116	Illumina PCR Primer Index 10 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATAG	20	0.006935096	52.513435	2
CTAGGAC	20	0.006935096	52.513435	3
TCGTTGT	20	0.006943024	52.49827	26
TTTCGGA	100	0.0	45.511642	1
GCTGGTT	40	4.5794673E-5	43.748558	13
CGAAACT	50	3.4645145E-6	41.998615	33
TCTTAAC	35	0.0012512419	40.010235	3
GGTTCTC	35	0.0012530211	39.99868	16
TGCTTGA	155	0.0	36.127842	55
GTATGCC	160	0.0	34.998844	42
CTGCTTG	165	0.0	33.938274	54
GTCTTCT	165	0.0	33.938274	49
CTTCTGC	170	0.0	32.94009	51
GCCGTCT	170	0.0	32.94009	46
TGCCGTC	170	0.0	32.94009	45
TGCCGGG	65	2.0942518E-5	32.306625	12
TCGTATG	175	0.0	31.998943	40
CTCGTAT	165	0.0	31.817131	39
TCTTCTG	180	0.0	31.110083	50
TTCGCCT	45	0.004299154	31.110083	29