FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335748
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT9120.27163229565030916No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5540.16500470591038519No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4660.13879457211956586No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4630.1379010448312425No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT4280.12747655980080297TruSeq Adapter, Index 6 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2100.034.99984742
ACCTTTA400.002417053334.9998477
CTCGTAT2000.033.24985539
AGTATCG851.0612348E-732.94103670
CGTATGC2300.031.95638541
CCGTCTT2300.031.95638547
TTATAGT552.9890498E-431.8180454
GTCTTCT1800.031.1109849
CTTGAAA2200.030.22714257
GCCGTCT2600.029.61525746
TCGTATG2250.029.5554340
TTAGACA604.976993E-429.1665424
GCTTGAA2350.028.29775256
TATGCCG2600.028.2691143
GTAATGG500.007203364327.9998829
TGAAAAA2700.027.22210759
GAATAAG657.933913E-426.9309841
CATAGGG1305.3823896E-926.9229622
TATAGTT657.947738E-426.9229625
AGGGGGG2500.026.59988670
Produced by FastQC (version 0.11.2)