FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481927
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT8750.18156276780508251No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6700.13902520506217747No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5760.11952017629226003No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5720.11869017506800823No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5630.11682267231344165No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5390.11184266496793083No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATG350.001254475940.0036772
ACTCTCA400.00241818534.99958421
GTTTATA450.004304066831.113972
AGTCATA450.00430625331.1107410
TATGCTT2350.026.8109745
ATATAAG1455.9299055E-1026.5541652
GTACTGA808.6550106E-526.252411
TAGGACT3750.025.2023164
TATATGC2650.025.0966453
ATCTGGG3800.024.8707087
TTGTCTC2400.024.79137210
CTAGGAC4100.024.7583733
CAGGGGT851.3075846E-424.7055914
CTTGTCT2600.024.2304829
ATGCTTG2900.024.140156
TATAAGA1451.722401E-824.140153
TACCATG4000.023.6247210
ATGTCTA2700.023.33305733
AATCAGG3750.023.33305536
AACGGGT3900.023.33305522
Produced by FastQC (version 0.11.2)