FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497759
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT11560.2322409037305202No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT8450.16976086821132316No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCA7090.142438408948909No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6550.1315897854182446No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC6010.12074116188758013No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5920.11893305796580272No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5800.1165222527367662No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT5740.1153168501222479No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTA5220.10487002746308957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGT200.006950009652.4994749
TAGGAAT200.006950009652.49947451
GAGCTTT450.0043063531.110811
TTGAGGG952.8169416E-729.4763492
TATTTAC500.007203323828.0025312
CAATACG500.007203323828.0025314
CTAGACA959.377298E-625.7918054
TCTAGTG700.001224931825.002268
TATACAT700.001224931825.002263
TATACCG2400.024.7939075
TGTAGGA1001.3957137E-524.5022142
GTCGTAA2300.024.34758234
ATACTAA1301.6039667E-724.23052840
GTGTAGG1301.6039667E-724.2305281
GTTAGAG1451.7245839E-824.13768862
TAATGCC1752.0008883E-1023.9997644
GTCATAT2500.023.799761
CCTAGGG901.9255556E-423.3354422
AGGGGCG901.9255556E-423.3354425
ATACTGG750.001831684523.33311
Produced by FastQC (version 0.11.2)