FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255566
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4450.1741233184382899No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4380.1713842999460022No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4240.16590626296142677No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4090.16003693762081028No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT3950.15455890063623487No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA3380.13225546434189211No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3360.1314728876298099No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3310.1295164458496044No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3110.12169067872878239No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2840.11112589311567266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAG252.3875081E-455.9999961
CTTCTAG305.8707775E-446.6666641
CTAGACA404.5847333E-543.754
ACCGTGT350.001253937540.08
TAGACAG552.9869913E-431.818185
TAAGGTG450.004302255331.11110910
GTATGCC1251.05501385E-1030.80000142
CATATGT952.810084E-729.4736821
GTCTTCT1202.2846507E-929.16666649
TATACCT604.973582E-429.1666665
TATGTTC853.9431743E-628.823533
CTCGTAT1103.1650416E-828.63636239
CCGTCTT1352.564775E-1028.5185247
ATGTTCC755.571328E-528.04
TAGACAT500.007200045527.9999985
TAGGGGT500.007200045527.9999984
AGGGGTG1154.8732545E-827.3913065
TATAGGG808.644807E-526.252
GCCGTCT1358.025381E-925.92592846
TACTATA700.001223945525.02
Produced by FastQC (version 0.11.2)