FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358157
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT7650.2135934799543217No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6100.1703163696367794No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5110.14267486046622013No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5060.1412788246495252No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4920.13736992436277945No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4890.1365323028727625No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4650.12983133095262692No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4420.12340956619583032No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT4100.11447493696898287No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT3880.10833237937552527TruSeq Adapter, Index 6 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3820.10665713639549136No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC3710.10358585759876256No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAA200.00694820452.49989729
ATATAAG1450.033.7930342
TCTGACG552.9894063E-431.8181178
CATATAA1700.030.8866041
TATAAGA1600.030.624943
AATACGC1600.030.6249421
AAGTAAT1052.0183506E-829.99993965
ATAAGAA1650.029.6969094
TGGGATG604.977584E-429.1666075
AATAGAC604.977584E-429.1666072
ATGTAAC500.007199007628.0038551
TAAGTTC500.00720391727.99994330
TCCTACA500.00720391727.9999432
TGTAGGA755.5775672E-527.9999432
ACTGGGT500.00720391727.9999438
CTCGTAT1653.6379788E-1227.575739
ACCGGTA1800.027.22216815
TTTCGGA1554.3655746E-1127.1005041
ATAGACC657.9486764E-426.9230233
TTCGGAA1850.026.4864332
Produced by FastQC (version 0.11.2)