FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356691
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT12330.34567735098446556No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT6350.17802523753052363RNA PCR Primer, Index 41 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5540.1553165064439529No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5300.1485879935294134No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4700.13176671124306472No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT3980.11158117249944631No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3980.11158117249944631No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA3570.10008662960377471No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAC200.006944768652.5064162
TACAGCG601.2131832E-535.0042762
GTTCACT500.00719951128.0034227
CAAGTGA500.007204441427.99949621
TGTACCG906.154074E-627.225555
GTATGCC3100.027.09628742
TAGGGTG1056.74745E-726.6699285
GTACCGA808.6470354E-526.2532086
GCCGTCT3350.026.11893346
CTCGTAT3150.025.55509439
GCTTGAA3300.025.45408656
AAATGTC851.3067084E-424.70543726
CTTGTAC1151.4914076E-624.3508033
TGCTTGA3450.024.34738755
TCTTCTG3600.024.30511750
CCGTCTT3600.024.30511747
TATGCCG3550.023.66154543
CTATTGG1202.1570431E-623.3394551
GTCTTGT1202.1570431E-623.3394551
TCTTGAA901.9236517E-423.3361852
Produced by FastQC (version 0.11.2)