FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518614
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC25480.49130952885961426No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC22880.44117590346577606No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC22560.4350056111096114No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC17930.3457291935813534No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC9830.1895436683159344Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT9490.1829877326875094No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA8910.17180407779196089Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC6690.12899767457106825No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG5400.10412368351027931No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCG459.19514E-538.888738
CATACCA400.002418262234.9998554
AACCATT450.0043063931.11098326
GACGTAA450.0043063931.11098318
AATTCAG450.0043063931.11098340
TTCGGAA1700.028.8261892
TTTCGGA1750.028.0052851
GTCCTAG1502.910383E-1128.0052851
AGTATAT500.007203530528.0025842
ATCCTTA500.007206922427.99988653
CCTAGTC500.007206922427.9998863
ATACTGC500.007206922427.9998866
ATATAGG500.007206922427.9998863
GGATTGA500.007206922427.9998864
CGACTCA500.007206922427.99988669
TATATGT1154.893809E-827.3911914
TATAGAA657.9491286E-426.925562
ACAGTGC657.9536083E-426.9229648
AGAGGTT959.385176E-625.78936865
ATGTTAT1659.640644E-1125.4544422
Produced by FastQC (version 0.11.2)