FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662058
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT28790.43485616063849386No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT15530.23457159342534945RNA PCR Primer, Index 41 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC12170.18382075286455266No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC11120.16796111518930365No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC8980.13563766316546283No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC7570.11434043543012846Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA7090.10709031535001465Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC6980.10542882949832191No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6920.10452256448830767No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT6660.10059541611157935RNA PCR Primer, Index 41 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGT207.921055E-570.009068
CTCGTAT6150.030.73104339
CTATAGG703.487347E-530.0016171
GTATGCC6300.029.99935342
GTCTTCT6500.027.99939749
GTAGTTT657.956754E-426.92249718
CGTATGC7050.026.80793241
GCTTGAA7050.026.80793256
TTACACA1207.385279E-826.255384
TGCTTGA6800.026.24943555
AGTAATG808.666495E-526.24943442
TCTCGTA7250.026.06840138
TATGCCG7400.026.01295343
CCGTCTT7150.025.94349547
CTTGAAA7450.025.8383757
ATGCCGT7500.025.66611344
GCCGTCT7250.025.58565346
TTGAAAA7750.025.28977658
TGCCGTC7650.025.16285545
TAATACC700.001224540825.0051274
Produced by FastQC (version 0.11.2)