FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524176
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC7860.14994963523701962No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT7750.14785110344617075No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC7240.13812154696132595No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC6350.12114251701718506No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC5500.10492658954244377Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT5380.10263728213424497No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAATA652.0969575E-532.3187032
GTCTTCT2300.031.95521449
GTATAAG450.00429916831.1217143
CTCGTAT2450.029.99877239
GTATGCC2350.029.78601642
CAATACT500.00719486828.009544
CCGTCTT2500.027.99885447
GCGTGTA500.00720828727.9988549
TATGCTT1554.3655746E-1127.1060095
CAGGGGT657.955429E-426.92197614
TATGCCG2700.025.92486643
TTCTATA959.360332E-625.7982622
TCTCGTA2850.025.7884238
ATGCCGT2900.025.34379244
TCTTCTG3100.024.83769450
ATCTTTT7050.024.8216812
CTTGAAA2850.024.560457
GCTTGAA3150.024.44344756
TCTTTTT7450.023.48897213
CATATAC750.001828078423.3412863
Produced by FastQC (version 0.11.2)