FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208392
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT5310.25480824599792695No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3130.15019770432646168No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2890.13868094744519943No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT2880.13820108257514685RNA PCR Primer, Index 41 (95% over 22bp)
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2810.13484202848477866No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2770.1329225690045683No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2440.11708702829283273No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAA200.006944090652.529
TATCCTA350.001253404240.05
TTAGATA459.1759815E-538.888894
AGGACCC400.00241490535.05
ATAAGAA703.4772856E-530.04
CACCGGT703.4772856E-530.014
TAATACT604.970465E-429.1666664
TTAAGAC500.007197037328.0000022
TATTAAG500.007197037328.0000022
GATTATG500.007197037328.0000027
ACCGGTA755.5664572E-528.015
CATATAA755.5664572E-528.01
CCGGTAA906.1438204E-627.22222316
GCGGGGA808.6372806E-526.2513
TAGATAC808.6372806E-526.255
AATACGC808.6372806E-526.2521
GTATGCC1358.007191E-925.92592642
ATCTTTT2700.025.9259267
CTCGTAT1101.0102285E-625.45454439
AATACTA700.00122318625.05
Produced by FastQC (version 0.11.2)