FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199100
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA4610.23154193872425918No Hit
CGTTGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTA3850.19337016574585636No Hit
ATCCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGACC2710.13611250627825214No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA2150.10798593671521849No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGT2030.10195881466599699No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG207.902853E-570.0173341
CCTAGGA150.002222227969.999752
GGTAACT200.00694373952.49981348
GTCCTAG200.00694373952.49981348
AGACGGT200.00694373952.4998136
GTGACGT200.00694373952.49981345
TGGTCAC200.00694373952.49981310
CGATCAC305.8677385E-446.666515
TCTATAT305.8677385E-446.66653
GATCTTC855.638867E-1141.1763236
ACTATGC350.001253292339.9998558
GTCGCAA350.001253292339.99985516
TCTCCAT350.001253292339.9998558
TATAATA350.001253292339.99985526
TAATGTG350.001253292339.9998555
TGTAGGG459.174709E-538.8887522
ATGTAGG601.2094562E-535.0086671
GTCTTAC400.002411716435.0086671
ACACCAT601.2115406E-534.9998746
GACCACT400.00241469134.99987463
Produced by FastQC (version 0.11.2)