FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779275
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT18440.23663020114850342No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA14800.18992011805845177No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG14550.18671200795611306No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA12810.16438356164383564No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT12410.1592505854800937No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT11920.15296268967950982No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC11280.1447499278175227No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC10890.1397452760578743No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA10000.12832440409354848No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG9820.12601456481986462No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT9500.12190818388887106No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG9260.1188283981906259No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA8820.11318212441050977No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT8720.11189888036957428No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA8610.11048731192454524No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC8500.10907574347951622No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT7960.1021462256584646No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA7890.10124795482980976No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGT657.9519517E-426.9260146
AGAAACG6350.024.80267167
AACGGCT6400.024.60890270
ATCTTTT15000.024.5026727
ACCGTTA1752.0008883E-1024.0026178
ATATAAG3400.023.6790522
TAAGACA901.9266715E-423.335884
TTACTCG750.001831340823.3358784
ATCTGGG5500.022.911597
CTTTTTT15900.022.2322779
ACTAGTT952.7782418E-422.1076763
GTATGCC2850.022.10483742
GACCGTT1755.2405085E-922.00247
AGTACTC800.00266469521.8773888
GGTTGCA7750.021.67700216
CTCGTAT2750.021.63594639
TCTTTTT16400.021.557238
GCAACGG7800.021.53804820
GAAACGG7400.021.28337368
TGCAACG7900.021.26541519
Produced by FastQC (version 0.11.2)