FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375356
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT9120.2429693411055105No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4790.12761218683063544No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4320.11509074052366286No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT3940.10496701797759994No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3810.10150363921184156No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAGT352.0803085E-550.018094
GTATATG350.001252458940.014471
TACACAG601.2113627E-535.012665
ATCTATG1357.2759576E-1231.1140737
ATCTGGG5600.030.0028577
ATCATAA604.967445E-429.1772161
TATGCCG1601.8189894E-1228.43641743
TAAGGGT500.0071915728.0101284
ACTACTA500.007205619527.99893629
CTCGTAT1403.929017E-1027.49895539
TCTACTC657.932517E-426.9328163
TCTACGG657.944879E-426.9256388
GATCTGG11550.026.6763116
GTATGCC1757.2759576E-1225.99900842
AGCGATG2300.025.86858270
CTGGGCA2350.025.3181889
TGGGCAG2650.025.09338610
ACATTGG700.00122243525.0090451
TTTGAGT700.001225287624.9990533
TATCTAC851.303521E-424.7148194
Produced by FastQC (version 0.11.2)