FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426794
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT8050.1886155850363407No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT7960.18650683936512696No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG6790.15909314563934826No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6600.15464134922234146No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA6200.14526914623916926No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5960.1396458244492659No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5930.138942909225528No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT5330.12488460475076968No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5320.1246502996761904No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5240.12277585907955595No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4760.11152921549974928No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4520.10590589370984597No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4380.10262562266573569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAT501.7065843E-435.0073246
AATAAGG400.002415253835.0073243
GTCTAGG1003.255991E-1035.0032231
GTGTATA400.002416642835.0032231
GTATGCC2200.033.4082542
CTAGGCC652.097936E-532.3144534
TAATGTG450.004301048331.1176225
AGTCTAA450.00430351631.1139761
GTATAAG450.00430351631.1139761
CTCGTAT2200.030.22651339
TCTAGGC703.480779E-530.0062793
TAGGGTT604.9730076E-429.1727714
GCCGTCT2400.029.16593646
TATGCCG2450.028.57071143
GCTTGAA2700.028.51780356
GTCCTAG1601.8189894E-1228.4401171
TCTGAGT500.007198011528.005868
TATGCTT1750.028.005865
CTGCTAG500.007202130728.0025791
TTGTCTC1750.027.99929610
Produced by FastQC (version 0.11.2)