FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637470
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT28760.451158485889532No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT14340.22495176243587933Illumina Paired End PCR Primer 2 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC14190.22259871052755423No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC13350.20942161984093371No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC11670.1830674384676926No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC8910.1397712833545108No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC7380.1157701538895948Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA6440.10102436193075753Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAG659.969881E-943.0979775
ACTTAGG552.986153E-431.828741
ATAATAC450.004296747531.1263183
GCACTAT450.004296747531.1263186
GTCTTCT6950.029.20686749
TCGTATG7350.029.0458640
CTCGTAT6450.028.75795239
GTATGCC7550.028.73998542
TGCTTGA7600.028.09040855
TATGCCG7900.027.46669243
CCGTCTT7650.026.9918347
TATATGG657.932743E-426.9362372
GCCGTCT7800.026.92144846
TTCTGCT7950.026.8537252
ATCTTTT11650.026.4506917
TCTTTTT10950.026.2228578
CTTGAAA8200.026.0350157
CGTATGC8400.025.83177241
TTGAAAA8600.025.63798358
TCTTCTG8200.025.60820650