Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adr3wtzt14s60.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 637470 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 56 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT | 2876 | 0.451158485889532 | No Hit |
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT | 1434 | 0.22495176243587933 | Illumina Paired End PCR Primer 2 (95% over 22bp) |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 1419 | 0.22259871052755423 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 1335 | 0.20942161984093371 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 1167 | 0.1830674384676926 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 891 | 0.1397712833545108 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 738 | 0.1157701538895948 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 644 | 0.10102436193075753 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATAGAG | 65 | 9.969881E-9 | 43.097977 | 5 |
ACTTAGG | 55 | 2.986153E-4 | 31.82874 | 1 |
ATAATAC | 45 | 0.0042967475 | 31.126318 | 3 |
GCACTAT | 45 | 0.0042967475 | 31.126318 | 6 |
GTCTTCT | 695 | 0.0 | 29.206867 | 49 |
TCGTATG | 735 | 0.0 | 29.04586 | 40 |
CTCGTAT | 645 | 0.0 | 28.757952 | 39 |
GTATGCC | 755 | 0.0 | 28.739985 | 42 |
TGCTTGA | 760 | 0.0 | 28.090408 | 55 |
TATGCCG | 790 | 0.0 | 27.466692 | 43 |
CCGTCTT | 765 | 0.0 | 26.99183 | 47 |
TATATGG | 65 | 7.932743E-4 | 26.936237 | 2 |
GCCGTCT | 780 | 0.0 | 26.921448 | 46 |
TTCTGCT | 795 | 0.0 | 26.85372 | 52 |
ATCTTTT | 1165 | 0.0 | 26.450691 | 7 |
TCTTTTT | 1095 | 0.0 | 26.222857 | 8 |
CTTGAAA | 820 | 0.0 | 26.03501 | 57 |
CGTATGC | 840 | 0.0 | 25.831772 | 41 |
TTGAAAA | 860 | 0.0 | 25.637983 | 58 |
TCTTCTG | 820 | 0.0 | 25.608206 | 50 |