FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396164
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT11590.2925556082834382No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC8670.2188487596046082No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC7820.19739299885905837No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC7740.19537363314183015No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT6320.15952989166102927Illumina Paired End PCR Primer 2 (95% over 22bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC5870.14817095950162054No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC5840.14741369735765997Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA5350.13504508233963713Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAC252.379002E-456.0481071
AGTAGAA400.002416328535.0035362
CTAGTGC501.7074504E-435.0035323
TTAGGTC400.002417825434.99911523
CATAACT400.002417825434.99911534
GCCGTCT3700.034.05319646
CTCGTAT3150.033.33249339
GTATGCC3650.032.60191742
TTTCGGA1400.032.527921
TATGCCG3750.031.73252943
GCTTAAT450.00430561831.11032722
AGTCTAT450.00430561831.11032754
ACCGTTT450.00430561831.11032712
CTTGAAA3850.030.9083157
GTCTTCT4000.030.62422649
GCTTGAA4100.029.87729556
TCGTATG3900.029.61463740
TCTTCTG4050.029.38197350
TGAACGG604.9755245E-429.1696135
TTCTGCT4300.028.48765452