Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adr3wtzt14s59.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 396164 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT | 1159 | 0.2925556082834382 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 867 | 0.2188487596046082 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 782 | 0.19739299885905837 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 774 | 0.19537363314183015 | No Hit |
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT | 632 | 0.15952989166102927 | Illumina Paired End PCR Primer 2 (95% over 22bp) |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 587 | 0.14817095950162054 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 584 | 0.14741369735765997 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 535 | 0.13504508233963713 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTTCTAC | 25 | 2.379002E-4 | 56.048107 | 1 |
AGTAGAA | 40 | 0.0024163285 | 35.003536 | 2 |
CTAGTGC | 50 | 1.7074504E-4 | 35.003532 | 3 |
TTAGGTC | 40 | 0.0024178254 | 34.999115 | 23 |
CATAACT | 40 | 0.0024178254 | 34.999115 | 34 |
GCCGTCT | 370 | 0.0 | 34.053196 | 46 |
CTCGTAT | 315 | 0.0 | 33.332493 | 39 |
GTATGCC | 365 | 0.0 | 32.601917 | 42 |
TTTCGGA | 140 | 0.0 | 32.52792 | 1 |
TATGCCG | 375 | 0.0 | 31.732529 | 43 |
GCTTAAT | 45 | 0.004305618 | 31.110327 | 22 |
AGTCTAT | 45 | 0.004305618 | 31.110327 | 54 |
ACCGTTT | 45 | 0.004305618 | 31.110327 | 12 |
CTTGAAA | 385 | 0.0 | 30.90831 | 57 |
GTCTTCT | 400 | 0.0 | 30.624226 | 49 |
GCTTGAA | 410 | 0.0 | 29.877295 | 56 |
TCGTATG | 390 | 0.0 | 29.614637 | 40 |
TCTTCTG | 405 | 0.0 | 29.381973 | 50 |
TGAACGG | 60 | 4.9755245E-4 | 29.169613 | 5 |
TTCTGCT | 430 | 0.0 | 28.487654 | 52 |