FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210194
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT5740.2730810584507645No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT2610.12417100392970302No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAG150.00222239869.999766
TGTAGGG252.3865684E-455.999812
AACGTTC200.006944267552.4998259
TGTCTTC200.006944267552.4998232
TCTTTGC200.006944267552.499828
GAATTGT200.006944267552.4998215
CCGTCTT350.0012534539.99986347
ATCTTTG350.0012534539.9998637
CCACTTA459.176437E-538.88875664
GTACTGG601.2098833E-535.0082131
ACACGAA400.002414992834.9998817
CAAGTAA400.002414992834.9998827
GTGGGTT400.002414992834.9998814
CTTCTGC400.002414992834.9998851
AATCGGA400.002414992834.9998819
CACTTAA501.7057746E-434.9998865
TGCCGTC400.002414992834.9998845
AAGTCAA400.002414992834.9998832
TCTGCGC708.655261E-734.9998788
ATCTGGC1650.033.939287
Produced by FastQC (version 0.11.2)