FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225667
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6100.2703097927477212No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT5450.2415062902418165No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG4780.21181652612034546No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA4600.20384017158024875No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4290.19010311653897113No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3600.15952709080193384No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3500.15509578272410232No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC3390.15022134383848768No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3230.1431312509139573No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC3000.13293924233494486No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC2960.13116671910381225No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2620.11610027163918515No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2590.11477087921583573No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT2560.11344148679248627Illumina Paired End PCR Primer 2 (95% over 22bp)
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2510.11122583275357052No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC2460.10901017871465478No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG2450.10856704790687163No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2450.10856704790687163No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA2420.10723765548352217No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA2340.10369260902125699No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2280.1010338241745581No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTCC200.00693953152.5101436
GTCAAAT459.179948E-538.88778346
ACTATAC852.5556801E-937.065983
ATCTTGG852.5556801E-937.065987
TACTATA852.5556801E-937.065982
CTGCTAG400.002410417435.014521
CGTAGAG400.002413040735.0067633
ACGTAAT400.002415666134.99900419
AGGAGTA501.7064245E-434.99925
CTCGTAT1206.548362E-1132.08241739
CCCTACC552.982507E-431.8243272
TACACCG450.004297132631.117125
ACGTTGC450.00430179731.11022658
TCGGACC450.00430179731.11022618
TTTGAGT450.00430179731.11022633
GTATGCC1251.03682396E-1030.7991242
CTTACAC802.4533183E-630.6309173
TTTCGGA703.468713E-530.0124451
TGCTTGA1301.6552804E-1029.6145455
CCGTCTT1301.6552804E-1029.6145447
Produced by FastQC (version 0.11.2)