Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adr3wtzt14s54.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 221717 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT | 933 | 0.42080670404163867 | No Hit |
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT | 438 | 0.197549127942377 | TruSeq Adapter, Index 7 (95% over 21bp) |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 321 | 0.14477915540982422 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 255 | 0.11501147859658935 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 238 | 0.10734404669015005 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 231 | 0.10418686884632211 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTTATA | 20 | 0.006944853 | 52.49966 | 11 |
TACTTCT | 30 | 5.86927E-4 | 46.66637 | 4 |
GTTATAG | 30 | 5.86927E-4 | 46.66637 | 12 |
GTCTTAC | 40 | 4.5707377E-5 | 43.76946 | 1 |
CTCGTAT | 175 | 0.0 | 41.99973 | 39 |
GTCTTCT | 175 | 0.0 | 41.99973 | 49 |
TCTAGCC | 45 | 9.178238E-5 | 38.888638 | 8 |
TAGTAGT | 45 | 9.178238E-5 | 38.888638 | 41 |
TGCTTGA | 190 | 0.0 | 38.683964 | 55 |
TGAAAAA | 195 | 0.0 | 37.692062 | 59 |
GTATGCC | 195 | 0.0 | 37.692062 | 42 |
TATGCCG | 200 | 0.0 | 36.749763 | 43 |
CCGTCTT | 200 | 0.0 | 36.749763 | 47 |
GCCGTCT | 200 | 0.0 | 36.749763 | 46 |
GTAGGAT | 40 | 0.0024153148 | 34.999775 | 3 |
TTATAGT | 40 | 0.0024153148 | 34.999775 | 13 |
TTGAAAA | 220 | 0.0 | 34.999775 | 58 |
TATAGTT | 40 | 0.0024153148 | 34.999775 | 14 |
AGTAGTG | 50 | 1.7061067E-4 | 34.999775 | 42 |
GCTTGAA | 210 | 0.0 | 34.99977 | 56 |