FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221717
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT9330.42080670404163867No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT4380.197549127942377TruSeq Adapter, Index 7 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3210.14477915540982422No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2550.11501147859658935No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2380.10734404669015005No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2310.10418686884632211No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATA200.00694485352.4996611
TACTTCT305.86927E-446.666374
GTTATAG305.86927E-446.6663712
GTCTTAC404.5707377E-543.769461
CTCGTAT1750.041.9997339
GTCTTCT1750.041.9997349
TCTAGCC459.178238E-538.8886388
TAGTAGT459.178238E-538.88863841
TGCTTGA1900.038.68396455
TGAAAAA1950.037.69206259
GTATGCC1950.037.69206242
TATGCCG2000.036.74976343
CCGTCTT2000.036.74976347
GCCGTCT2000.036.74976346
GTAGGAT400.002415314834.9997753
TTATAGT400.002415314834.99977513
TTGAAAA2200.034.99977558
TATAGTT400.002415314834.99977514
AGTAGTG501.7061067E-434.99977542
GCTTGAA2100.034.9997756