FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349294
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT8480.24277542700418558No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6200.17750090181909794No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5880.16833956495101549No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5390.1543112678717642No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4930.14114184612389563No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4400.12596838193613402No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3950.11308525196539304No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT3830.10964975063986213TruSeq Adapter, Index 7 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3830.10964975063986213No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3580.10249245621167269No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTATC150.002222576470.0081567
TAATGTG150.002222576470.0081565
TATAGCG200.006944802652.506124
TCGTCTA200.006948736552.49860456
TATGGAG350.001253849740.0046655
GTCTTCT2200.039.7716749
GTATGCC2250.038.88785642
GCTTGAA2300.038.04246556
CTCGTAT2350.037.2330539
AAAGGGG2350.035.7437368
TGCTTGA2600.034.9990755
CCGTCTT2700.033.7028147
GCCGTCT2800.032.49913846
TAACCTA450.0043019431.1147375
ACTCGTA450.0043019431.1147376
AACCTAT450.0043019431.1147376
TACCGGT450.004304955731.11028330
TATGCCG2850.030.70093743
ATGCCGT3100.030.48305944
CGTATGC2900.030.17161241
Produced by FastQC (version 0.11.2)