FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200919
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC77880.6485033545143345No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC71010.5912971649211979No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC69420.5780573044476772No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC55860.46514377739048185No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC29590.24639463610784743Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA27450.2285749496843667Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC19120.15921140393315453No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG15850.13198225692157423No Hit
GTGCAGGGAGACGTGTGCTCTTCCGATCTGTGCAGGGAGACGTGTGCTCT13900.1157446921898979Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCC350.001254432540.008814
TTTCGGA2250.038.9006921
ACACCGT652.1009335E-532.3148046
GTCCTAT450.00430214631.1205521
AACTAGT450.00430214631.1205521
TATTTGC450.00430916531.11018669
CGATTTA450.00430916531.11018614
TTCGGAA2900.030.1815722
ACTAGTA500.007199823428.0084972
TGCTTGA2500.026.59920955
GCTTTAA700.001224418925.0075871
GGGTACA851.3093323E-424.70514716
AGCATAC750.001829846223.3404122
GGTACAC901.9293313E-423.3326417
AACGCAA750.00183339623.33263870
CTTGAAA3000.023.33263857
AAATGAA750.00183339623.33263855
GACACTC77000.023.09022370
TTGAAAA3050.022.95013658
GGACCGT2004.3655746E-1122.7550096
Produced by FastQC (version 0.11.2)