FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452734
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT14770.3262401321747428No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT7750.1711821952846484TruSeq Adapter, Index 7 (95% over 21bp)
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT6000.13252815118811487No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT6000.13252815118811487No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA5320.11750829405346186No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG4740.10469723943861076No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAT350.001253872440.007071
CGATCTA10800.035.013925
CGATCTG25600.034.056515
TTCCGAT61600.033.8202672
CCGATCT61550.033.5064164
TCCGATC62700.033.1710853
TCTGTCC751.4858524E-632.6760528
CTTCCGA64300.032.3929751
CTCGTAT4150.032.04677639
GATCTAG4100.031.5979296
AGTAATA450.00430666131.10973563
CTGTGCG802.4650944E-630.6236469
TCTTCTG4350.030.57336250
GTATGCC4350.030.57336242
GATCTGG13100.030.4701336
TCCTAGG604.967996E-429.1782672
TCTACTC604.9712113E-429.1750458
TTCTAAT604.974428E-429.1718221
GCATGTT604.980867E-429.16537920
TCTGTGC2050.029.0327288
Produced by FastQC (version 0.11.2)