FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500729
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT12190.2434450571067384No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11070.22107766875894946No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT7970.15916793315346225No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC7470.14918249192677077No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG7440.14858336545316927No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA7360.14698569485689864No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA7200.14379035366435736No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT7150.1427918095416882No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC7080.1413938477699514No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA7020.14019559482274843No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT6780.1354025830339365No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT6070.12122325649203461No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG5860.1170293711768242No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT5720.11423344763335057RNA PCR Primer, Index 47 (95% over 22bp)
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG5620.11223635938801227No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT5390.10764305642373419No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA5350.10684422112559888No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA5260.1050468417047944No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5050.100852956389584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTA1150.039.5687146
CTATACA601.2144415E-535.0030984
TTCCGAT42000.033.4196242
CCGATCT41700.033.324294
TCCGATC42250.033.304723
GCCGTCT2750.033.0905346
CTCGTAT2650.033.0184939
CTTCCGA43150.032.691191
GCTTGAA3000.031.4996456
TATATAC450.004304298731.1138613
GTATGCT450.00430640331.11075623
GTATGCC2950.030.84710742
TGCTTGA3400.029.852655
GATCTGG9300.029.7338126
GTCTTCT3200.029.53091249
ATCTGTG1551.8189894E-1229.3574357
CGATCTG20750.029.1833045
TAGACGT604.9777445E-429.1692474
CCGTCTT3150.028.8885647
GGATACA500.007203431328.0024782
Produced by FastQC (version 0.11.2)