FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345182
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10060.29144045749778374No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT5340.15470099831393294RNA PCR Primer, Index 47 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATC200.00694452452.5065232
CTCGTAT2350.035.74394239
ACGTAGA400.002412191135.0144961
CTCTAGG400.002412191135.0144961
GATACAC501.706763E-435.004353
TAGTGTG400.002415623535.004357
TCGTATG2800.031.24935240
GAACGTA450.00430476231.11046818
GTATGCC2700.031.11046642
TCTTCTG2800.029.99937850
GCCGTCT2850.029.47307446
ACTATAC604.9735367E-429.170293
GTCTTCT3000.029.16606149
ATATGCT500.00720421927.9994231
TCTCGTA3000.027.9994238
AAAGGGG2900.027.75804768
TGAAAAA3050.027.54041359
GTCTTAC1403.9108272E-1027.5113871
CGTATGC3150.026.66611541
CTTGAAA3150.026.66611557
Produced by FastQC (version 0.11.2)