FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347010
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT8150.2348635486009049No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT4630.13342554969597417RNA PCR Primer, Index 47 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3730.10748969770323623No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTATA501.7041039E-435.0136952
TAGGCCG501.7041039E-435.0136955
GGTCCAA601.2144472E-534.9985611
ACTATAC652.0942034E-532.3203353
GTAGGAT552.9823268E-431.8306313
TAATTGT552.9823268E-431.8306314
GTTTATA604.9658207E-429.178082
TTACACA604.9658207E-429.178084
CTATGGT500.00718979628.0109564
TAGGATA500.00718979628.0109564
ACGACTT500.00720499127.99884849
TCGTAAT657.949993E-426.92196835
GTATGCC2350.026.80740742
GTCTTAC1056.747705E-726.6694131
CTCGTAT2400.026.2489239
AAGAATT808.65599E-526.24891916
GTCTTCT2500.025.19896349
ATACTAA700.001225123224.99897240
TACTAAT700.001225123224.99897241
CGTATGC2550.024.70486541
Produced by FastQC (version 0.11.2)