FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326560
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10980.33623223909848116No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT6210.1901641352278295RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT3390.10380940715335618RNA PCR Primer, Index 47 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTACT305.873886E-446.66554331
TTCTAAT350.001250823140.023551
CGAACTA350.001254597939.9990429
TCTAGGC552.9865344E-431.8222898
ATAATAG450.004301270531.1151283
TATACTC450.004304495631.11036337
GTCTAAC604.959452E-429.1838381
AGAATAA604.9772765E-429.16596433
TTTCGGA1850.028.3950861
AAGAATA500.007203777327.99932732
GGGCAAA500.007203777327.99932711
GATCTTT8650.026.3039726
GTATGCC3200.025.15564542
TCTAGGG1401.1827069E-825.0032253
GATCTGG8700.024.9457476
CTTTTTT8150.024.9073759
CTCGTAT3100.024.83811239
GTCTTCT3250.024.76863349
GTACATG851.3010067E-424.7204281
CTGAGCT851.3064314E-424.7052889