Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adr3wtzt14s39.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 158714 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT | 930 | 0.5859596506924405 | No Hit |
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT | 417 | 0.262736746600804 | Illumina PCR Primer Index 11 (95% over 22bp) |
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT | 198 | 0.12475269982484218 | Illumina PCR Primer Index 11 (95% over 24bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCTTACC | 20 | 0.006941056 | 52.5 | 45 |
GCTAGGA | 20 | 0.006941056 | 52.5 | 11 |
CTTAGGT | 20 | 0.006941056 | 52.5 | 23 |
CTAGGAG | 20 | 0.006941056 | 52.5 | 12 |
GACGTGG | 35 | 2.0792748E-5 | 49.999996 | 49 |
GAACATT | 30 | 5.8640144E-4 | 46.666664 | 11 |
CTCTTCA | 35 | 0.0012525019 | 39.999996 | 33 |
TATAGTT | 35 | 0.0012525019 | 39.999996 | 5 |
GTATGCC | 185 | 0.0 | 37.837837 | 42 |
TATGCCG | 200 | 0.0 | 36.750004 | 43 |
TGCCGTC | 185 | 0.0 | 35.945946 | 45 |
TAGCCTG | 50 | 1.7038609E-4 | 35.000004 | 56 |
TGCTTGA | 190 | 0.0 | 35.0 | 55 |
TCTTAAC | 40 | 0.0024131762 | 35.0 | 3 |
CGTATGC | 205 | 0.0 | 34.146343 | 41 |
ATGCCGT | 205 | 0.0 | 34.146343 | 44 |
CTTGAAA | 195 | 0.0 | 34.102566 | 57 |
GTCTTCT | 165 | 0.0 | 33.939392 | 49 |
CCGTCTT | 200 | 0.0 | 33.250004 | 47 |
GCCGTCT | 200 | 0.0 | 33.250004 | 46 |