FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456334
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT7580.16610640451949668No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6340.13893332515219114No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5750.12600419867903773No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5540.12140230620554242No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC4830.10584352689039168Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4820.10562438915355857No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT459.193857E-538.888466
ATACGTG459.193857E-538.888467
ATATAGG851.0617077E-732.9443741
CTAAGAC552.9889442E-431.821273
TACAGGC703.48397E-530.0029132
TAAGACA604.97681E-429.1694984
TACGTGT604.9800036E-429.16630268
TATATCG500.007202469328.0027185
GGGTACC500.00720632427.99965111
GGGTTAC500.00720632427.99965122
GAGTATA657.947433E-426.925691
ACTATTA1455.9117156E-1026.55433
GTATGCC1951.8189894E-1225.12788842
CTATAAG700.001224727825.0024263
ACCACGC700.001224727825.0024268
GTACTAT2300.024.350191
TAAGGTA1652.6484486E-923.33565
TACTATT1502.467641E-823.3355982
TGTAGGA750.001830311123.3355982
ATCTTTT10850.023.228067
Produced by FastQC (version 0.11.2)