FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adr3wtzt14s36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adr3wtzt14s36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471451
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6330.13426633944991104No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5730.12153967220347396No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5670.12026700547883025No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4920.10435867142078391No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT552.99085E-431.8180874
CCTAGGG604.979975E-429.166582
CTACACT959.382729E-625.7893964
TCTTTTT8200.023.90236713
GTTCTGA750.001829207823.3382131
GGTAGGA901.9265649E-423.3332652
ATCTTTT8850.023.33326312
CTTTTTT8600.023.19760514
TATACAG1253.0856972E-622.3999335
CTATACA1304.3360433E-621.5383974
TACGACG3300.021.2120575
TCTGGGG1656.778646E-821.2120578
CTCGTAT2151.1459633E-1021.16272739
CCTAGAT2151.1459633E-1021.1627273
GAGGTAT1003.9289653E-420.99993737
GGATTAT1356.0013754E-620.7450791
TACACTG1356.0124075E-620.7406795
GATCTTT10150.020.68959211
AGTTAGA1205.7086658E-520.41660561
GACGTAA1558.727038E-720.3225218
Produced by FastQC (version 0.11.2)